ABSTRACT
Valproic acid (VPA), an anti-epileptic drug, has been reported to cause male sub/infertility. Together with searching for alternative treatments, the degrees to which testosterone levels and sperm quality are decreased under VPA treatment also need to be clarified. This study aimed to investigate the protective effects of Momordica cochinchinensis (MC) aril extract containing antioxidant capacity on adverse reproductive parameters induced with VPA. Rats were divided into 6 groups (control, VPA, 200 mg kg-1 of MC only, 50, 100, 200 mg kg-1 MC+VPA, respectively, n=8 in each). Animals were pretreated with MC extract for 23 days before co-administration with VPA (500 mg kg-1, i.p.) for 10 consecutive days. All reproductive parameters including histology, and expression of androgen receptor (AR), Ki-67, tyrosine phosphorylated proteins, and steroidogenic proteins in testis were examined. The results showed that MC could prevent all reproductive parameters in VPA-treated rats. Moreover, MC+VPA groups showed significant declining of testicular histopathologies compared to VPA group. It also decreased the malondialdehyde level and changes of the testicular StAR, AR, and tyrosine phosphorylated protein expressions. In conclusion, M. cochinchinensis aril extract can prevent adverse male reproductive parameters and essential testicular proteins damages induced with VPA.
Se ha informado que el ácido valproico (VPA), un fármaco antiepiléptico, causa infertilidad masculina. Junto con la búsqueda de tratamientos alternativos, los grados a los que los niveles de testosterona y la calidad del esperma son disminuidos bajo el tratamiento de VPA también necesitan ser aclarados. El objetivo de este estudio fue investigar los efectos protectores del extracto aril de Momordica cochinchinensis (MC) que contiene capacidad antioxidante sobre parámetros reproductivos adversos inducidos con VPA. Las ratas se dividieron en 6 grupos (control, VPA, 200 mg kg-1 de MC solamente, 50, 100, 200 mg kg-1 de MC + VPA, respectivamente; n = 8 en cada uno). Los animales fueron pretratados con extracto de MC durante 23 días antes de la coadministración con VPA (500 mg kg-1, i.p.) durante 10 días consecutivos. Se examinaron todos los parámetros reproductivos, incluyendo la histología, y la expresión de receptor de andrógenos (AR), Ki-67, proteínas fosforiladas con tirosina y proteínas esteroidogénicas en los testículos. Los resultados mostraron que MC podría prevenir todos los parámetros reproductivos en las ratas tratadas con VPA. Además, los grupos MC + VPA mostraron una disminución significativa de las histopatologías testiculares en comparación con el grupo VPA. También disminuyó el nivel de malondialdehído y los cambios de las expresiones testiculares de las proteínas StAR, AR y tirosina fosforiladas. En conclusión, el extracto de aril de M. cochinchinensis puede prevenir los parámetros reproductivos masculinos adversos y los daños esenciales de proteínas testiculares inducidos con VPA.
Subject(s)
Animals , Male , Rats , Testis/drug effects , Plant Extracts/administration & dosage , Valproic Acid/toxicity , Momordica/chemistry , Antioxidants/administration & dosage , Phosphoproteins , Immunohistochemistry , Receptors, Androgen/drug effects , Blotting, Western , Rats, Wistar , Ki-67 AntigenABSTRACT
This study aimed to investigate the antioxidative activity and antimutagenicity of six extracts of three plants in Annonaceae, collected from Khok Phutaka area, Amphur Phuwiang, Khon Kaen. The results showed the strong antioxidative activities of the extracts from stem-root of Ellipeiopsis cherrevensis and leaf of Polyalthia evecta with EC₅₀ at 14.3 and 14.7 \µg/ml, respectively. The other extracts with relative order of activity were leaf of E. cherrevensis \> leaf of P. debilis \> stem-root of P. evecta \> stem-root of P. debilis (EC₅₀ = 17.9, 22.8, 46.3 and 177.9 \µg/ml, respectively). The plant total phenolic contents are well correlated with their antioxidative activities. For pre-incubation bacterial mutation test, the extracts from leaf and stem-root of E. cherrevensis showed mutagenic effect on Salmonella typhimurium TA98 and TA100 in the absence of S-9 mix. Whereas in the presence of S-9 mix, the mutagenicity was found in the extracts from leaf of E. cherrevensis, leaf and stem-root of P. debilis and leaf of P. evecta. The antimutagenicity was detected in both TA98 and TA100 in all extracts in the presence of S-9 mix. In contrast, in the absence of S-9 mix only the extracts from stem-root and leaf of P. debilis; and leaf of P. evecta showed the antimutagenicity to TA98. Therefore, these three plants are interesting to be further studied on their anticancer activity.